Ultrasensitive Electrochemical Immunoassay for Avian Influenza Subtype H5 Using Nanocomposite

Ultrasensitive Electrochemical Immunoassay for Avian Influenza Subtype H5 Using Nanocomposite

We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The graphene oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in...

Phylogenetic study of the recent outbreak of Avian Influenza H5 subtype in Turkeys, Iran, 2019

Background: Global epizootic distribution of HPAI H5N8 (Clade 2.3.4.4) in poultry and wild birds was demonstrated after 2010. HPAI virus is a major concern not only in birds and poultry industry but also in global human health. Wild migratory birds and their linked to backyard birds are playing a critical role in spreading HPAI and the creation of genetic reassortment. Methods: In this study i...

Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA.

Nucleic acid sequence-based amplification (NASBA) is a technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique has been developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The a...

Comparison of serological assays for detecting antibodies in ducks exposed to H5 subtype avian influenza virus

BACKGROUND Chicken red blood cells (RBCs) are commonly used in hemagglutination inhibition (HI) tests to measure hemagglutinating antibodies against influenza viruses. The use of horse RBCs in the HI test can reportedly increase its sensitivity when testing human sera for avian influenza antibodies. This study aims to compare the proportion of positives detected and the agreement between two HI...

Isolation and Identification of Avian Influenza Virus H9N2 Subtype